mc00376 Guide to the Mary-Dell Chilton Papers, 1947 - 1999 MC 00376 Finding aid prepared by Processed by: Nathan Crowe and Stephanie Horowitz; machine-readable finding aid created by: Stephanie Horowitz Special Collections Research Center 2006 This finding aid was produced using the Archivists' Toolkit 2011-10-29T22:29-0400 Finding aid written in English. Mary-Dell Chilton Papers MC 00376 Special Collections Research Center 7.75 Linear feet 1947 - 1999 The Mary-Dell Chilton Papers include laboratory records, correspondence, notes, manuscripts, reprints, articles, and photographs from 1947 through 1999 documenting Chilton's career as a plant geneticist. The bulk of the records are from the late 1970s and early 1980s. Mary-Dell Chilton has worked as a plant geneticist and genetic engineer throughout her career at the University of Washington, Washington University in St. Louis, and at Syngenta Biotechnology, Inc. in Research Triangle Park in North Carolina. For current information on the location of these materials, please consult the Special Collections Research Center Reference Staff. 17 archival boxes Chilton, Mary-Dell, 1939- Restrictions to Access

This collection is open for research; access requires at least 24 hours advance notice.

 Acquisitions Information

Gift of Mary-Dell Chilton, 2005 June (Accession number 2005-0005)

 Preferred Citation

[Identification of item], Mary-Dell Chilton Papers, MC 376, Special Collections Research Center, North Carolina State University Libraries, Raleigh, NC

 Copyright Notice

The nature of the NCSU Libraries' Special Collections means that copyright or other information about restrictions may be difficult or even impossible to determine despite reasonable efforts. The NCSU Libraries claims only physical ownership of most Special Collections materials.

The materials from our collections are made available for use in research, teaching, and private study, pursuant to U.S. Copyright law. The user must assume full responsibility for any use of the materials, including but not limited to, infringement of copyright and publication rights of reproduced materials. Any materials used for academic research or otherwise should be fully credited with the source.

 Processing Information note

Processed by Nathan Crowe and Stephanie Horowitz, 2006 August

Encoded by Stephanie Horowitz, 2006 August

 Biographical Note

Mary-Dell Chilton was born in Indianapolis, Indiana, on February 2, 1939. After receiving a B.S. in chemistry from the University of Illinois, Urbana, Mary-Dell Chilton continued to study at the University of Illinois, completing her Ph.D. in Chemistry in 1967 with a dissertation entitled Transforming Activity in Single Stranded DNA from Bacillus Subtilis. She then went on to complete post-doctoral fellowships at the University of Washington, Seattle, until 1971, when she received a faculty position at the same university. Chilton remained at the University of Washington until 1979 when she accepted a faculty position at Washington University in St. Louis. Chilton's research teams at the universities first demonstrated that the genes responsible for causing disease could be removed from the bacterium without adversely affecting its ability to insert its own DNA into a plant cell. Chilton and her collaborators at Washington University were also the first to produce transgenic plants.

In 1983, Mary-Dell Chilton left Washington University and accepted a position in the private sector at CIBA-Geigy Corporation, which later became Syngenta Biotechnology, Inc. (SBI) in Research Triangle Park, North Carolina. Her tenure there spanned both research and administrative roles, including vice president of agricultural biotechnology, distinguished science fellow, and principal scientist. In 2002, Chilton's contributions to the development of plant biotechnology earned her the Benjamin Franklin Medal in Life Sciences from the Franklin Institute. In 2011 she received the Presidential Award from the Crop Science Society of America.

Chilton's current research (as of September 2011) is directed at improving the technology for introducing new genes into plants. Specifically her work has involved gene stacking (keeping all genes in one place) and targeting (getting genes to insert at a particular place).

Mary-Dell Chilton was married to Dr. Scott Chilton, who was a professor of botany in the College of Agriculture and Life Sciences at North Carolina State University from 1983 to 2004. In 2004, a few weeks before Scott Chilton's untimely death, Mary-Dell Chilton and her husband completed plans to create an endowment supporting the collections of the NCSU Libraries.

 Scope and Content Note

The Mary-Dell Chilton Papers include laboratory records, correspondence, notes, manuscripts, reprints, articles, and photographs, 1947-1999, documenting Chilton's career as a plant geneticist. The bulk of the records are from the late 1970s and early 1980s.

 Organization of the Collection

The Mary-Dell Chilton Papers are organized into six series: Laboratory Records, Research, Publications and Manuscripts, Teaching and Advising, Correspondence, and Subject Files.

 Collection Arrangement

The series have been ordered roughly by significance and extent. Most folder titles have been maintained as originally labeled. Within series, folders have been arranged alphabetically by folder title.

 Related Material Scott Chilton Papers, MC 375, Special Collections Research Center, NCSU Libraries Chilton, Mary-Dell, 1939- Biotechnology Letters (correspondence) Manuscripts (document genre) Plant genetics--Research--North Carolina Publications Laboratory Records Series 1 2.5 Linear feet 1962 - 1981 5 archival boxes Scope and Contents note

This series contains Mary-Dell Chilton's laboratory notebooks and other records from her laboratory experiments. Many were originally contained in binders labeled by experiment or subject. The records contain a great deal of information regarding DNA experimentation and other genetics experiments.

 Brute Force Experiments, Folder 1 of 2 1 1 1976 Summer Brute Force Experiments, Folder 2 of 2 1 2 1976 Summer Brute Force Experiments II 1 3 1976 Summer Clones - Plasmid/Plant 1 4 1978 Corn 1 5 1968 Crown Gall 2 1 1973 - 1975 Digests - Randall Saiki 2 2 1978 - 1979 DNA/RNA on HA. I. B. subtilis 2 3 1969 DNA/RNA on HA. II. Unique Mouse, Mouse Genome Fractionation 2 4 1969 DNA/RNA on HA. II. Unique Mouse, Mouse DNA/RNA on HA 2 5 1969 Graduate Laboratory Work 2 6 1962 - 1963 M-13 Cloning/DNA Sequencing 2 7 1981 Methods 3 1 undated Mouse 3 2 1970 March 27 - 1970 August Nopaline Plasmids, Cryptopine Plasmids 3 3 1978 - 1979 Notes 3 4 1979 Octopine - Nopaline, Book 2, Folder 1 of 2 3 5 1977 Octopine - Nopaline, Book 2, Folder 2 of 2 3 6 1977 Plasmid DNA Isolation, Restriction Endonuclear Experiments 3 7 1976 Plasmid Genetics; Transformation 3 8 1975 September 17 - 1976 March 27 Plasmids - Common Bond Experiments - Cloning 4 1 1977 - 1978 Radioactivity Log (Archives) 4 2 undated "Randy Papers" - Enzyme Preps., Plasmid Clones, Alkaline Gels, DNA Concentrations, Plasmid Mapping, Misc., Methods 4 3 1978 - 1979 Sequencing 4 4 1978 - 1979 Tomato Satellite 4 5 1972 October 16 - 1974 January 24 Gel Films [oversize] 17 1 undated DNA Digests and Eco Plants [oversize] 17 2 1978 - 1979 Research Series 2 2.25 Linear feet 1947 - 1982 5 archival boxes Scope and Contents note

This series includes material related to Mary-Dell Chilton's research on plants, plant genetics, genetic engineering, and other subjects in which she was professionally interested. It contains articles and reprints written by other scientists, as well as many of Chilton's own research notes. The series also includes professional activity information related to Chilton's research, such as grant applications and information on professional conferences attended by Chilton.

 5B and 0B Maize 5 1 1968 - 1973 ACS [American Cancer Society] Proposal 5 2 1974 ACS [American Cancer Society] Grant Application, Molecular Basis of Crown Gall Tumor Genesis 5 3 1980 March 25 Agrobacterium References 5 4 1980 American Association for the Advancement of Science Annual Meeting Program 5 5 1979 January B. subtilis Transformation 5 6 1969 - 1970 Blattner, Structure of the Constant...Heavy Chain Messenger RNA 5 7 1979 December 14 Cloned Tumor DNA Binder, Notes and Articles 5 8 1969 - 1975 Cloning Origins 5 9 1978 Cloning Technology 5 10 1976, 1978 Corn 5 11 1947 - 1973 Development of a Tumor-Inducing Plasmid Vector for Plant Genetic Engineering, "Unfinished Symphony," Folder 1 of 2 5 12 1980 - 1982 Development of a Tumor-Inducing Plasmid Vector for Plant Genetic Engineering, "Unfinished Symphony," Folder 2 of 2 5 13 1980 - 1982 DNA Articles 5 14 1979, undated DNA-RNA Hybrid 5 15 1960 - 1969 DNA Sequencing 5 16 undated DNA Transformation Articles 6 1 1958 - 1964 DNA Transformation Articles 6 2 1965 - 1966 DNA Transformation Articles 6 3 1967 - 1968 DNA Transformation Articles 6 4 1969 - 1971 DNA Transformation Articles 6 5 1972 - 1975 DNA Transformation Meetings 6 6 1967 - 1968 DNA Transformation Notes 6 7 1961 - 1973 Drug Resistance Gene Sequence 6 8 1979 - 1982 EMBO [European Molecular Biology Organization] Meeting, Molecular Biologists Look at Green Plants 6 9 1980 Farley-Chilton Enterprises, Inc. Grant and Budget Information 6 10 1980 - 1981 Genetic Engineering 7 1 1980 - 1981 Genetic Engineering Articles 7 2 1978 July George Jen Proposal, In Vivo Suppression of Plant Nonsense Mutants Award Application 7 3 1981 May 29 Gordon Conference on Plant Molecular Biology 7 4 1980 Grant Application, Molecular Basis of Crown Gall Tumorigenesis 7 5 1975 March 26 Images and German Articles 7 6 undated Insulin Genes - Howard Goodman 7 7 1979 Ken Barton - Data 7 8 undated Ken Barton - Data, Plasmids and Bugs Freezer Collection 7 9 undated Ledoux 7 10 1975 - 1979 Mapping Cuts in Clones 7 11 1980 September 1, undated Mapping Cuts in Clones 7 12 undated Mapping Cuts in Clones, BAM 3A and 6, ECO 18 and 26 7 13 undated Mapping Cuts in Clones, BAM 14A and BAM 9 7 14 1982 March 3, undated Mapping Cuts in Clones, Biohazards, Draft of T37 Map Paper 7 15 undated Mapping Cuts in Clones, BSTE II Digests of Deletion Mutants, Folder 1 of 2 7 16 undated Mapping Cuts in Clones, BSTE II Digests of Deletion Mutants, Folder 2 of 2 7 17 undated Mapping Cuts in Clones, Cosmid Clones 7 18 undated Mapping Cuts in Clones, SMA Digests of Deletion Mutants 7 19 undated Mapping Cuts in Clones, T37 Map, Raw Data, Rough Draft Maps 7 20 undated Meetings 7 21 1981 Summer Michael Kahn, Construction of a Cosmic Gene Bank... Proposal 7 22 1979 October 25 Northerns Graphs and Papers 7 23 1977, undated PhD Thesis Research Binder 8 1 1965 - 1966 PhD Research, Dubnau, Gene Conservation 8 2 1965 September PhD Research Expression, Article 8 3 1958 October 3 PhD Research, Fate of Donor DNA 8 4 1960 - 1965 PhD Research, Integration - Genetics Studies 8 5 1963 - 1964 PhD Research Uptake 8 6 undated Photographs 8 7 undated Plant Satellite DNA 8 8 1968 - 1977 Plastid DNA Papers 8 9 1975 - 1977 Recombinant DNA, Public 8 10 1976 - 1978 Recombinant DNA Research Proposal 8 11 c. 1978 Renatured Mouse Satellite DNA Paper 8 12 1973 Renatured Mouse Satellite DNA Paper 8 12 1973 Restriction Endonucleases Purification, Methods 8 13 1976 - 1979 Restriction Enzyme Isolation 8 14 1978 Restriction Enzyme Isolation, Chilton et al., Edinburgh preprint Notes 8 15 undated Restriction Enzyme Isolation, Yadav Grant Applications Notes 8 16 undated Schell Preprints Reprints 8 17 1976 - 1978 Seminar Notes 9 1 1979 - 1980 Simon Silver Mechanisms of Plasmid-Determined Resistance Mercury... 9 2 c. 1979 Tomato 9 3 1973 - 1975 Translocatable Elements 9 4 1977 United States/Australia Science Foundation Grant Application 9 5 1981 University of Minnesota Symposium, Emergent Techniques for the Genetic Improvement of Crops, Notes 9 6 1979 March Publications and Manuscripts Series 3 0.5 Linear feet 1965 - 1982 1 archival box Scope and Contents note

This series includes Mary-Dell Chilton's publications in various forms, including manuscripts, preprints, and reprints. It also includes supporting records when the creator had originally included such records with the file. Thus, some research notes are contained in this series rather than in the Research series. The series includes publications written solely by Chilton as well as some publications written jointly with other scientists.

 Agrobacterium Tumefaciens 10 1 undated Agrobacterium Ti Plasmids As a Tool for Genetic Engineering in Plants 10 2 1980 Bevan and Chilton Manuscript Research and Notes 10 3 1981, undated Bevan and Chilton Northern Manuscript, Multiple Transcripts of T-DNA... 10 4 undated Bridgehead Dehydrohalogenation in the Photodimer of 9-chloroanthracene 10 5 1965 Detection and Quantitation of Octopine in Normal Plant Tissue and in Crown Gall Tumors 10 6 1974 February DNA from Maize with and without B. Chromosomes: A Comparative Study 10 7 1973 August Fingerprints of Agrobacterium Ti Plasmids 10 8 1978 Foreign DNA...is Transcribed in Crown Gall Tumors 10 9 1977 October 6 Genetic and Base Sequence Homologies in Bacilli 10 10 1969 January 14 Highly Conserved DNA...Overlaps T-DNA Maintained in Plant Tumors 10 11 1978 September 14 Hybridization of Ribonucleic Acid with Unique Sequences of Mouse Deoxyribonucleic Acid 10 12 1972 Nuclear CplMt Manuscript T-DNA from Agrobacterium Ti Plasmid... 10 13 1980 On the Question of the Integration of...Bacterial DNA into Plant DNA 10 14 1975 July On the Question of Integration of...Deoxyribonucleic Acid by Tomato Plants 10 15 1975 December Restriction Endonuclease Analysis of Virulence Plasmids From Agrobacterium Tumefaciens 10 16 1978 Restriction Endonuclease Mapping of a Plasmid... 10 17 1978 Ribosomal DNA in a Nuclear Satellite of Tomato 10 18 1975 Search for Bacterial DNA in Crown Gall Tumors 10 19 1977 Short Direct Repeats Flank the T-DNA on a Nopaline T. Plasmid 10 20 1982 October Site-Specific Insertion of Genes Into T-DNA... 10 21 1981 Stable Incorporation of Plasmid DNA into Higher Plant Cells 10 22 1977 June Studies of the T-DNA of Agrobacterium Ti Plasmids 10 23 1978 T-DNA of a Crown Gall Teratoma is Covalently Joined to Host Plant DNA 10 24 1980 October 2 Tailoring the Agrobacterium Ti Plasmid as a Vector for Plant Genetic Engineering 10 25 1981 Theoretical Explanation of Mouse Satellite DNA Renaturation Kinetics 10 26 1973 November 7 Transforming Activity in Both Complementary Strands of Bacillus subtilis DNA 10 27 1967 August 18 Transforming Activity in Single-stranded DNA from Bacillus subtilis 10 28 1968 Yadav, et al., Covalent Joining 10 29 1980 Yadav, et al., Short Direct Repeats Flank the T-DNA and Supporting Research, Folder 1 of 4 10 30 1980 - 1982 Yadav, et al., Short Direct Repeats Flank the T-DNA and Supporting Research, Folder 2 of 4 10 31 1980 - 1982 Yadav, et al., Short Direct Repeats Flank the T-DNA and Supporting Research, Folder 3 of 4 10 32 1980 - 1982 Yadav, et al., Short Direct Repeats Flank the T-DNA and Supporting Research, Folder 4 of 4 10 33 1980 - 1982 Yadav, et al., Short Direct Repeats Flank the T-DNA and Supporting Research, J. Vanderleyden 10 34 undated Teaching and Advising Series 4 1.25 Linear feet 1959 - 1999 3 archival boxes Scope and Contents note

This series includes readings and lecture notes from courses that Mary-Dell Chilton taught in conjunction with her career in academia. It also contains several theses written by Chilton's advisees.

 Bio 572, Seminar in Plant Biology, Readings #1, 9 articles 11 1 1981 Fall Bio 572, Seminar in Plant Biology, Readings #2 11 2 1981 Fall Bio 572, Seminar in Plant Biology, Readings #3-4, 8 articles 11 3 1981 Fall Bio 572, Seminar in Plant Biology, Readings #5, 4 articles 11 4 1981 Fall Bio 572, Seminar in Plant Biology, Readings #6, 5 articles 11 5 1981 Fall Bio 572, Seminar in Plant Biology, Readings #7, 6 articles 11 6 1981 Fall Biology Honors Thesis - "Construction of a pAMI-agrocin Hybrid Plasmid Using Recombinant DNA Methods," Douglas M. Brugge 11 7 1982 March 15 EMBO [European Molecular Biology Organization] Course Material "The Use of Ti-Plasmid as Cloning Vector for Genetic Engineering in Plants" 11 8 undated Equipment Grant for Bio 437 11 9 1981 Micro 503 Lab Manual 11 10 undated Micro 510 Articles, DNA Transformation 12 1 1963 - 1971 Micro 510 Competence Lecture, Papers and Notes, Folder 1 of 3 12 2 1959 - 1973 Micro 510 Competence Lecture, Papers and Notes, Folder 2 of 3 12 3 1959 - 1973 Micro 510 Competence Lecture, Papers and Notes, Folder 3 of 3 12 4 1959 - 1973 Micro 510 References, Notes, Articles 12 5 1972 - 1975 T-Even Phage Lecture, Folder 1 of 2 12 6 1970 - 1971, undated T-Even Phage Lecture, Folder 2 of 2 12 7 1970 - 1971, undated Thesis - Excision and Transposition of Tn5 and Other Transposons, Carol Ann Egner 13 1 1981 December Thesis - Light Modulation of PEA Fed-1 Translation Depends on its 5' End, Eric Ralph Hansen 13 2 1999 Thesis - Virulence Plasmid Found in Avirulent Mutants, Margaret Palmquest 13 3 1976 June 10 Correspondence Series 5 0.75 Linear feet 1963 - 1983 2 archival boxes Scope and Contents note

This series includes Mary-Dell Chilton's correspondence, which is mainly professional in nature, though some contains a personal element. It also contains a small number of related records that were contained within a correspondence file by the creator.

 [Armin] Braun Letters 14 1 1978 Chapter for Molecular Biology of Plant Tumors 14 2 1981 Chronological Labeled "Micro" 14 3 1967 - 1970 Chronological, Outgoing Labeled "1973 Vintage" 14 4 1972 - 1974 Crown Gall, Cucumber Satellite 14 5 1971 - 1972 DNA Transformation, Charles Laird 14 6 1966 June 27 Dubnau, D. 14 7 1967 - 1969 Genetics Department 14 8 1963 - 1967 [Jeff] Schell Letters 14 10 1976 - 1978 Letters, Folder 1 of 3 14 11 1980 - 1983 Letters, Folder 2 of 3 14 12 1980 - 1983 Letters, Folder 3 of 3 15 1 1980 - 1983 [Martin] Drummond 15 2 1978 Nestor Sabbatical 15 3 1974 [R. A.] Schilperoort Letters 15 4 1978 Subject Files Series 6 0.5 Linear feet 1977 - 1981 1 archival box Scope and Contents note

This series contains files maintained by Mary-Dell Chilton regarding her employment search as well as records containing information only indirectly related to her career.

 Academic Appointment [Washington University] 16 1 1979 Computer Information 16 2 1980, undated Curriculum Vitae 16 3 1978 July Departmental Seminar Program 16 4 1980 - 1981 Diagram - Plasmids 16 5 undated French Lessons and Notes 16 6 1979, undated Job Applications, Folder 1 of 2 16 7 1978 Job Applications, Folder 2 of 2 16 8 1978 Kotty Postle Correspondence, Grant and Fellowship Application 16 9 1977 - 1978 Revisions of NIH [National Institute of Health] Guidelines on Experiments 16 10 1977 November 2 Using the Computer 16 11 1981, undated